Phages that attack American Fould Brood – field test

It must have been the first time that three people ever stood around a beehive and said “American Foul Brood. Great!”  No, we’re not mad, nor gluttons for punishment.  Professor Penny Amy, a microbiologist, and researcher Diane Yost, an environmental microbiology student  (both of University of Nevada, Los Vegas), and I, the beekeeper of the sadly afflicted hive, were about to test rehydradted phages that will hopefully kill the bacteria that cause AFB.

The images in this blog are from two different treatments.  Some are from when Penny and Diane were here; some are from the most recent treatment, which I did just the other day.

Background

University of Las Vegas AFB phage researchers in WA beeyard

Professor Penny Amy and Researcher Diane Yost

 

 

 

 

 

 

 

 

 

I “met” Penny and Diane while doing a search for anyone in the world who was doing work on for my blog post about AFB research.   What I found was that there is almost no work being done! One fellow in Denmark has retired.  I couldn’t contact a German researcher.  This left a UK program about how AFB is distributed and the vital research being conducted by Penny and Diane.

They are working to isolate phages that specifically target and kill AFB bacteria and find a means of delivering these phages to infected (and potentially infected) honeybee colonies.  (Phages are virus that attack and kill bacteria).   They are currently in the testing stage – early days, but promising.  Again, the above link has more information on their work.

How It Was Done

I had two hives that had been tested by the USDA Bee Lab in Maryland and had come back positive for American Foul Brood.

At the University of Nevada, Los Vegas, Penny and Diane had isolated phages that attack AFB – in the lab.

Diane had discovered a way to freeze-dry them (there is a real fancy word for this, but, alas, I forgot it); then restore them to fighting strength.

Some of these phages came from a sample I sent, others came from beekeepers throughout the US and in Europe.

These phages were in their kit.

Kit for testing phages that attack AFB

The Kit

 

 

 

 

 

 

 

I made up some cane sugar syrup (about 1.5 sugar to 1 water, I think).  I didn’t weigh or measure my syrup contents, so a sample of this syrup went back with Penny and Diane to be analyzed.  I did use “city of Glacier” water, rather than the rain capture water I usually use, because I figured the city water would be free of maple and cedar oils.

At the hives, Penny and Diane rehydrated the phages in the test tubes that housed the phages.

Phages that attack AFB in Vials

Phages before and after rehydration

 

 

 

 

 

 

 

 

 

My syrup was used to rehydrate the phages, but it was later decided that this stage was best done with store-bought water (that old “what’s in the water” question around here).  Indeed, the water worked well during the most recent treatment.

The rehydrated phages were then added to 400 milliliters my syrup in a spray bottle.

Spray bottle and vial of phages that attack AFB

Spray Bottle Marked at 400 ml

 

 

 

 

 

 

 

 

 

This mix was sprayed onto the bees working the infected comb and onto the comb itself.

One hive would be the “Control” hive: just syrup, applied in the same manner as the treatment, but with no phages.

A second hive would be the “Treated” hive (they have a better word, I’m sure, but this is an AFB hive that gets phage/syrup mix).

A third, AFB-free hive, would also be “Treated”.   This hive is called the Non-Infected Hive, but I think of it as the “Karen is overjoyed to have these phages in yet another one of her bee yards” hive.

Each of the three hives is in a separate location, each separated by over 4 miles.

At each step, photos, notes and samples were taken.  Samples included soil, beeswax, royal jelly, and anything that looked interesting.  Frames were numbered.  Box numbers noted.

Bags being marked for AFB soil & wax samples

Marking The Sample Bags

 

 

 

 

 

 

 

Soil samples from bee yard with AFB

Soil Samples from bee yard

 

 

 

 

 

 

 

Clarifications

Words like rehydrate are mine – I’m sure both Penny and Diane would have a much better term.

Spraying on brood versus feeding:  The idea is to get the nurse bees to eat the phage/syrup mix and then deliver it to the larvae.

If it were fall, I think it could be put in the feed.  But during a good honey flow the bees would rather eat honey than syrup.  Thus direct spray was the final decision.

The First Hive : The Control Hive

The first hive we went to showed no visible signs of AFB.  I felt bad!  There was an intense honey flow near that hive when we were there.  Blackberries and clover were in abundance.  If I didn’t know the test had shown positive for AFB, I would not have believed the hive was infected.  It was picture perfect brood.  Diane later tested a sample from this hive and AFB doesn’t show!  Yet it tested positive before, and had been slated for killing/burning/scorching.  I still think AFB will show up again.

Control frame in American Foul Brood Phage Test

Control frame, looking too good.

 

 

 

 

 

 

American Foul Brood seems to rear its ugly head in times of low forage, be that a dearth, or the fall and winter, here.  I think it’s a combination of bees getting stressed, while consuming every last bit of food, some of which might have AFB – which the bees then pass on to their young.

This first hive was chosen to be the “Control” hive. Penny said that this worked out well.  As they wanted the first hive we visited to be the “Control” (no phages, just syrup), and the second hive to be the “Treated” hive.

By doing the “Control” first, we would not carry phages, on our equipment or ourselves, to the control hive.  Had phages contacted the “Control”, it would no longer be a control.

The Second Hive : AFB Infected Hive

The second hive was the “American Foul Brood. Great!” hive.

Frame from phage test against American Foul Brood

American Foul Brood Hive before most recent treatment

 

 

 

 

 

 

This sad little hive had shown a few AFB cells when I had checked it about a week before.  I was worried then that there wasn’t enough AFB to conduct a decent Field Test.  I need not have worried.  The AFB had spread.

The hive was sprayed in the same manner as the first hive.

The above picture was taken just before the most recent phage treatment.  Two weeks before the entire frame was covered in AFB affected cells.  Now eggs and larvae can be seen amid the AFB – we’ll see what happens with those young in two weeks (the next treatment).

The Third Hive : The Non-Infected Hive

This hive was treated and photographed in the same manner as the Control and Treatment hives.

Future Treatments:

I get to continue treating the second and third hive, and spraying the first, control, hive with sugar syrup.  I’m pretty excited about this.  What could be better (other than not having AFB)?  I love science.  I love to experiment.  And I think if this phage treatment works it will be one of the best things to hit beekeeping in a long, long time.

Early Days:

It is early days.  But I’m going to hope, while Penny and Diane do all the work.  It would be wonderful if we could drag AFB treatment out of the 19th century and into the 21th century.

That’s the news from Brookfield Farm Bees And Honey in Maple Falls, Washington.  I’m writing this while making Joint and Muscle Salve and First Aid Salves.  You know what they say, a “Watched Pyrex Container of Infused Oils and Beeswax Never Melts”

What’s happening in your bee yards? The heat here is amazing – after heavy rains last week.  The weather’s just going to keep us guessing.

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About brookfieldfarmhoney

Brookfield Farm, a small off-grid apiary in Maple Falls, WA focuses on the beauty and bounties of Washington’s wilderness. I sell honey from our bees, whose naturally-treated, antibiotic-free hives are home to bees who fly Washington’s mountains and farmlands. Herbal salves and lip balms from Brookfield beeswax. Delicately infused honeys and vinegars. Varietal honeys from independent Washington beekeepers. Karen Edmundson Bean: beekeeper, photographer. Her love of the wilderness inspires her to discover new ways of bringing the wonders of nature to others. Brookfield Farm : the tastes, textures, sounds, and images of nature.
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21 Responses to Phages that attack American Fould Brood – field test

  1. slideridge says:

    Reblogged this on Slide Ridge Bee Notes and commented:
    This is very interesting, and good to see that at least someone is working on an effective treatment for a real problem!

    • I find it very exciting – It’s early days, but if they can make this work, it will be a whole new world for beekeeping. I’m just really pleased that my misery of dealing with AFB might be able to help.

  2. Emily Heath says:

    Very interesting! What is the usual treatment in your area for AFB, would all the affected hives usually be burnt?

    • Hi Emily – For folks like me (natural treatments, no antibiotics) the usual treatment is to kill all the bees, then burn all the frames (honey, brood, pollen, every frame in the hive). Then take what I term a “garden blow torch” – attached to a propane tank – and scorch the inside of the boxes, tops, and bottoms. But I’ve read that this only kills 85% (or something like that) of the AFB bacteria.

      I’m afraid the majority of folks put antibiotics in the hive. This, as you know, does not kill the bacteria. Then they give their bees antibiotics forever (or as you said in your blog, prophylactically – I like that word), while having a box of AFB bacteria to share with near-by beekeepers. Labs in the US have now produced a 4th antibiotic in the US for AFB.

      • Emily Heath says:

        What you do is the UK way of doing things too. AFB must be reported to a national bee inspector, who will come and destroy the hive for you. Giving antibiotics against AFB is illegal. I’m glad you don’t use those.

        • I wish that the US would stop using the amount of antibiotics we do on all animals. It’s a major mess. And with bees, as you know, it doesn’t solve the problem – it just keeps the bees alive (on twice a year antibiotics) while having hives full of AFB. I hope that the information Penny and Diane got from the field test will help in their work to isolate and reproduce these phages… Interestingly Diane told me that the strongest phages they had came from my hives and a hive in Belgium — where the chemicals and antibiotics aren’t used either. So it would seem that beekeepers here have been killing – or at best – harming the very phages that can attack AFB. How ironic.

  3. Robert says:

    Very interesting.I look forward to future posts!

  4. Steve Miller says:

    Karen I was so glad to meet you in person today ( Steve M. The high way guy) I have enjoyed your site so much. Very informative and a big help to me Thanks.

  5. Pingback: Participating in a Bee Health Survey | Brookfield Farm Bees & Honey

  6. Pingback: Karen Bean - Off grid beekeeper and blogger extraordinaire - KM036

  7. Percy Kugarakuripi says:

    Thank you so much for periodic updates on apiculture information.Would you please assist in idenifying an institute where i can go and further my knowledge on apiculture production. I am a provincial apiculturist in zimbabwe. Currently my qualification is just a diploma in horticulture. My intention is to venture into commercial production since we have all natural resouces required to carry out production. To date we have registered two associations which are producing processing natural honey.

    regards

    Percy

    On 7/12/13, Brookfield Farm Bees & Honey Blog

  8. Steve says:

    Have you tried Borage Oil (Borago Officinalis) to treat AFB… A German study found that Borage Pollen in a cell protected brood all around it from AFB. AFB and Human Stomach Ulcer bacteria both look almost identical under a microscope and Borage Oil was an effective treatment to kill the bacteria that causes stomach ulcers in humans. It is surmised that both are stomach bacteria and Borage oil should be an effective treatment but little or no testing has been done to determine its effectiveness. I use Borage oil in my hives and have never had AFB but until I get AFB and try Borage Oil as a treatment I cant be sure. Would love to have some testing done to determine its effectiveness.

    • I like that idea. Perhaps I’ll add it to the girls mix – after a bit more research. I am apparently the only person in this area who is incapable of growing Borage. To everyone else it’s a weed – I’ve tried seeds, I’ve tried individual starts, I’ve tried potted transplants – the individual starts almost made it, then died at the height of summer (must have been all of 80F)… So I really like the idea of the oil…thank you

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